환경시료은행 환경시료 채취, 초저온 균질화 표준운영절차 개선사항 제안

Environmental Specimen Banks (ESBs) have been known as a system to collect and homogenize and preserve environmental samples cryogenically for long-term monitoring of bio-accumulation of environmental pollutants. In Korea, National Environmental Specimen Bank (NESB) was established in 2009 and its Standard Operating Procedures (SOPs) had been prepared during the period of 2010～ 2012. In this study, we dealt with the applications of the SOPs for the 7 kinds of specimens (shoots of Red pine (Pinus densiflora Siebold & Zuccarini) and Korean pine (Pinus koraiensis Siebold & Zuccarini), leaves of Mongolian oak (Quercus mongolica Fischer) and Zelkova tree (Zelkova serrata Makino), eggs of Feral pigeon (Columba livia var. domestica Linnaeus), muscles and organs of Common carp (Cyprinus carpio Linnaeus), and Freshwater bivalve (Unio (Nodularia) douglasiae Griffith & Pidgeon) in 2012. The SOPs were reviewed based on the findings regarding the sample size, period, sampling methods etc.. In addition, the criteria of cryogenic homogenization (particle size ≤ 200μm) were tested in terms of milling run time and frequency.